Protein Sequence Digestion

Paste a protein/peptide sequence or import a FASTA file. Select the digestion enzyme and digestion parameters. Click Run Digestion.

100% local processing. All calculations run entirely in your browser using plain JavaScript. No sequence data is ever transmitted, uploaded, or sent to any server or third-party service. This tool makes no network requests after the page loads.
① Protein Sequence
② Digestion Enzyme
③ Cysteine Modification
④ Parameters
Minimum peptide length
4

Peptides < 4 AA will be excluded

Missed cleavages
0

No missed cleavages (fully digested)